INFLUENCE OF EXPLANTS, NAA AND BAP CONCENTRATIONS ON IN-VITRO CALLUS INDUCTION IN CUCUMBER VARIETIES
Keywords:callus weight, cytokinin, auxin, explant
In vitro callus induction in cucumber varieties as influenced by explants and different concentrations and combinations of NAA and BAP was studied. Three cucumber varieties viz. Shila, Green Field (GF) and Shital; three types of explant viz. leaf, node and internode; three NAA concentrations viz. 1.0, 2.0 and 3.0 mg/l and three BAP concentrations viz. 0.0, 1.0 and 2.0 mg/l were used in this investigation. Maximum number of callus was obtained from variety Shital (5.59) followed by Green Field (5.01) and Shila (4.19), respectively. Different explants showed significant difference in all the parameters studied but internodal explants were superior over other two explants. Internodal explants took minimum time (10.53 days) for callus induction whereas leaf segments took maximum time (15.38 days). The internodonal explants of variety GF produced the highest weighted (3.49 gm) callus when they were interacted with 3.0 mg/l NAA and 2.0 mg/l BAP in MS medium and the same variety also produced the lowest weighted (0.99 gm) callus when it’s nodal explants cultured in MS medium supplemented only with 3.0 mg/l NAA.
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