INFLUENCE OF EXPLANTS, NAA AND BAP CONCENTRATIONS ON IN-VITRO CALLUS INDUCTION IN CUCUMBER VARIETIES

Authors

  • S M FAISAL Horticulture Division, Bangladesh Agricultural Research Institute, Khagrachari Hill District, Bangladesh.
  • MD SHAHIDUL HAQUE Department of Biotechnology, Bangladesh Agricultural University, Mymensingh, Bangladesh.
  • KHONDOKER MD NASIRUDDIN Department of Biotechnology, Bangladesh Agricultural University, Mymensingh, Bangladesh.

Keywords:

callus weight, cytokinin, auxin, explant

Abstract

In vitro callus induction in cucumber varieties as influenced by explants and different concentrations and combinations of NAA and BAP was studied. Three cucumber varieties viz. Shila, Green Field (GF) and Shital; three types of explant viz. leaf, node and internode; three NAA concentrations viz. 1.0, 2.0 and 3.0 mg/l and three BAP concentrations viz. 0.0, 1.0 and 2.0 mg/l were used in this investigation. Maximum number of callus was obtained from variety Shital (5.59) followed by Green Field (5.01) and Shila (4.19), respectively. Different explants showed significant difference in all the parameters studied but internodal explants were superior over other two explants. Internodal explants took minimum time (10.53 days) for callus induction whereas leaf segments took maximum time (15.38 days). The internodonal explants of variety GF produced the highest weighted (3.49 gm) callus when they were interacted with 3.0 mg/l NAA and 2.0 mg/l BAP in MS medium and the same variety also produced the lowest weighted (0.99 gm) callus when it’s nodal explants cultured in MS medium supplemented only with 3.0 mg/l NAA.

References

Bangladesh Bureau Statistics (BBS) (2006): Monthly statistical bulletin-Bangladesh. – Bangladesh Bureau of statistics, Ministry of Planning, Government of the People’s Republic of Bangladesh, Dhaka, Bangladesh 55p.

Chandra, R. (2003): Comprehensive micropropagation of horticultural crops. – IBDC 344p.

Chawla, H.S. (2002): Introduction to Plant Biotechnology. – CRC Press 760p.

Cullins, C.A. (1991): Breeding for resistance to physiological stress. In: advanced methods in plant breeding and biotechnology. – CAB International Redwood Press 11p.

Huda, S., Islam, R., Bari, M.A., Asaduzzaman, M. (2003): Shoot differentiation from cotyledon derived callus of chickpea (Cicer arietinum L.). – Plant Tissue Cult. 13 (1): 53-59.

Islam, R., Sarkar, K.P., Naderuzzaman, A.T.M., Joarder, O.I. (1994): In vitro regeneration of plants from cotyledons of Momordica charantia L. – Plant Tissue Cult. 4(2): 105-109.

Kim, S.G., Chang, J.R., Cha, H.C., Lee, K.W. (1988): Callus growth and plant regeneration in diverse cultivars of cucumber (Cucumis sativus L.). – Plant Cell, Tissue and Organ Culture 12(1): 67-74.

Lou, H., Kako, S. (1995): Effects of auxin combinations on somatic embryogenesis in Cucumis sativus L. – Journal of the Japanese Society for Horticultural Science 64(3): 571-579

Lou, H., Kako, S. (1994): Somatic embryogenesis and plant regeneration in cucumber. – HortScience 29(8): 906-909.

Murashige, T., Skoog, F. (1962): A revised medium for rapid growth and bio assays with tobacco tissue cultures. – Physiologia Plantarum 15(3): 473-497.

Nabi, S.A., Rashid, M.M., Al-Amin, M., Rasul, M.G. (2002): Organogenesis in teasle gourd (Momordica dioica Roxb.). – Plant Tissue Cult. 12 (2): 173-180.

Nonnecki, I.L. (1989): Vegetable Production. – Van Nostrand Reinhold, New York 670p.

Peirce, L.C. (1987): Vegetables. Characteristics, production, and marketing. – John Wiley and Sons 448p.

Shibli, R.A., Ajlouni, M.A. (1996): In vitro propagation and Ex vitro acclimatization of ‘Sahara F1’cucumber (Cucumis sativus L.). – Plant Tissue Cult. 6(2): 95-98.

Torres, K.C. (1989): Tissue Culture Techniques for Horticultural Crops. – Van Nostrand Reinhold 224p.

Wang, W., Vinocur, B., Altman, A. (2003): Plant responses to drought, salinity and extreme temperatures: towards genetic engineering for stress tolerance. – Planta 218(1): 1-14.

Downloads

Published

2022-10-14

How to Cite

FAISAL, S. M., HAQUE, M. S., & NASIRUDDIN, K. M. (2022). INFLUENCE OF EXPLANTS, NAA AND BAP CONCENTRATIONS ON IN-VITRO CALLUS INDUCTION IN CUCUMBER VARIETIES. Quantum Journal of Engineering, Science and Technology, 3(3), 20–30. Retrieved from https://qjoest.com/index.php/qjoest/article/view/69

Issue

Section

Articles